术苦芩总多糖对湿热泄泻仔猪小肠杯状细胞数量以及MUC-2和ITF-3 mRNA转录的影响

doi:10.11843/j.issn.0366-6964.2019.06.021

摘要/Abstract

摘要： 为探讨术苦芩总多糖（ZKQPs）对湿热泄泻仔猪小肠修复作用的影响，将60头断奶仔猪分为空白对照组（n=10）造模组（n=50），造模组采用高温高湿环境配合高脂配合饲料等建立湿热泄泻模型，进一步将造模成功后的50头仔猪随机分为模型组、阳性药物组（白头翁散）、ZKQPs高、中、低剂量组（n=10），分别拌料给药，连用7 d。采用过碘酸雪夫染色（PAS）结合显微镜观察小肠组织杯状细胞（GC），利用RT-PCR和ELISA分别检测湿热泄泻仔猪小肠黏液中黏蛋白-2（MUC-2）和肠三叶因子（ITF-3）的转录和含量变化。试验结果：1）模型组仔猪十二指肠、空肠、回肠中GC数量均减少，与空白组比较，差异显著（P<0.05）；与模型组比较，ZKQPs高剂量组、中剂量组和阳性药物组仔猪十二指肠、空肠、回肠中GC数量均增加，ZKQPs高剂量组、阳性药物组仔猪十二指肠、空肠、回肠和ZKQPs中剂量组仔猪十二指肠、回肠GC的数量差异显著（P<0.05）。2）与空白组比较，模型组仔猪十二指肠、空肠、回肠中MUC-2 mRNA的转录量和含量均降低，差异显著（P<0.05或P<0.01）。与模型组比较，ZKQPs各剂量组和阳性药物组仔猪十二指肠、空肠、回肠中MUC-2 mRNA的转录量和含量均升高，ZKQPs高剂量组和阳性药物组仔猪十二指肠、空肠、回肠MUC-2 mRNA的转录量和含量差异显著（P<0.05或P<0.01）。3）与空白组比较，模型组仔猪十二指肠、空肠、回肠中ITF-3 mRNA的转录量和含量均降低，差异极显著（P<0.01）。与模型组比较，ZKQPs高剂量组、中剂量组和阳性药物组仔猪十二指肠、空肠、回肠中ITF-3 mRNA的转录量和含量均升高，ZKQPs高剂量组、中剂量组和阳性药物组仔猪空肠、回肠中ITF-3 mRNA的转录量和含量差异显著（P<0.05或P<0.01）。ZKQPs可提高湿热泄泻仔猪小肠GC的数量以及MUC-2和ITF-3 mRNA的转录，促进湿热泄泻仔猪小肠内GC分泌物MUC-2和ITF-3分泌。

Abstract: To investigate the effect of Polysaccharides from ZhuKuQin (ZKQPs) on the recovery function in damp-heat diarrhea Piglet intestine, 60 piglets were divided into blank control group (n=10) and damp-heat diarrhea model group (high temperature, humidity environment combined with high fat compound feed), and 50 model piglets were randomly divided into model group, positive drug group (Pulsatilla powder), ZKQPs high, middle and low dose group, with 10 piglets in each group, drugs were mixed with feed, for 7 days. Small intestinal goblet cells (GC) were stained with PAS and observed by Microscope, RT-PCR and ELISA were used to detect the transcriptional quantity and content of mucoprotein-2 (MUC-2) and intestinal trilobular factor (ITF-3) in intestinal mucus of damp-heat diarrhea piglets. The results were as follows:1)The number of GC in duodenum, jejunum and ileum of piglets in model group decreased significantly when compared with that in blank group (P<0.05); The number of GC in duodenum, jejunum and ileum of piglets in ZKQPs high, middle dose group and positive drug group increased. The number of GC in duodenum, jejunum and ileum of piglets in the ZKQPs high dose group and the positive drug group were significantly different when compared with the model group (P<0.05), and the number of GC in duodenum and ileum of piglets in ZKQPs middle dose group was significantly different (P<0.05). 2)The transcriptional quantity and content of MUC-2 in duodenum, jejunum and ileum of piglets were decreased in model group, and the difference was significant when compared with that of blank group (P<0.05).The transcriptional quantity and content of MUC-2 in duodenum, jejunum and ileum of piglets in each ZKQPs dose groups and positive drug groups increased. The transcriptional quantity and content of MUC-2 in duodenum, jejunum and ileum of piglets in ZKQPs high dose group and positive drug group were significantly different from those in model group (P<0.01). 3)The transcriptional quantity and content of ITF-3 in duodenum, jejunum and ileum of piglets were decreased in model group, and the difference was significant when compared with that of blank group (P<0.05). The transcriptional quantity and content of ITF-3 in duodenum, jejunum and ileum of piglets in ZKQPs high, middle dose group and positive drug group increased.The transcriptional quantity and content of ITF-3 in jejunum and ileum of piglets in ZKQPs high, middle dose group and positive drug group were significantly different from those in model group (P<0.01). ZKQPs can increase the number of GC and the transcription of MUC-2 and ITF-3 mRNA in small intestine of damp-heat diarrhea Piglets, and promote the secretion of MUC-2 and ITF-3 in small intestine of damp-heat diarrhea Piglets.

中图分类号:

S853.74

林春发, 郝永峰, 刘娟. 术苦芩总多糖对湿热泄泻仔猪小肠杯状细胞数量以及MUC-2和ITF-3 mRNA转录的影响[J]. 畜牧兽医学报, 2019, 50(6): 1301-1311.

LIN Chunfa, HAO Yongfeng, LIU Juan. Effect of Polysaccharides from ZhuKuQin on the Number of Intestinal Goblet Cell and the Transcription of MUC-2 and ITF-3 mRNA in the Small Intestine of Damp-heat Diarrhea Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(6): 1301-1311.

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